|Team Affiliations||Doctor Octopus, Chameleon, Scorpion, Shocker, Rhino, Mysterio (deceased), Vulture (replaced Mysterio)|
The Kingpin sprang the six supervillains from prison, as he required their services to finally rid himself of Spider-Man. Kidnapping May Parker, the Six hoped to get Parker to lead Spider-Man into a trap. After a battle with a powerless Spider-Man, they unmasked him. Believing Peter to be a fake, they again used him in hopes of luring Spider-Man.
After they were unsuccessful in defeating Spider-Man, they spilt up and concentrated on their own, solo efforts. When Kingpin devised a new plan for world domination, he once again called on the six, replacing the late Mysterio with The Vulture. After helping Fisk locate the six keys required for the Doomsday Weapon and helping create Electro, the Six again split after The Kingpin was almost killed by the master of electricity, never to reunite again.
In the comics, the team was called the Sinister Six but was changed in this show because the network felt that sinster was too scary of a word for a kid's show.