- Otto Octavius: The most well known user of the name. Little is known about him besides that he fought Spider-Man.
- Doctor Octopus (Alternate Universe): An alternate version of Otto who fought another Spider-Man.
- Doctor Octopus (Spider-Woman Universe): Yet another version of Otto who fought Spider-Woman.
- Olivia Octavius: A scientist at Alchemax and the only known female version. It is unknown how she was related to Otto. She used inflatable arms rather than Otto's mechanical ones. She was killed during a battle with various Spider-Men.
Otto fought with Spider-Man many times. Miles Morales had an action figure of the Otto Octavius version of Doctor Octopus in his dorm room.
Olivia used her powers while working at Alchemax. She fought with several versions of Spider-Man while attempting to create an interdimensional portal for Kingpin. During a fight with numerous Spider-Men she was hit by a train her portal brought in and presumably killed.